Study on subculture conditions of YTB mature embryos of indica rice callus

To establish the most optimal callus subculture system on YTB mature embryo,the effect of different callus subculture conditions on YTB mature embryo were studied.The results showed that NB medium was more suitable to the growth of callus subculture; YTB callus from mature embryos wasn't suitable for the second subculture.Fe iron concentration had a significant impact on callus subculture;hydrolysis of casein protein,proline,glutamine and sodium thiosulfate improved the quality of callus and reduced the browning rate;ascorbic acid(VC) had no significant impact on the embryo callus subculture growth and anti-browning.The consistency of morphology and cytology observation proved that exogenous putrescine(30 mg/L) in the callus subculture maintained callus in a good embryogenic condition,thereby enhanced the subsequent genetic transformation rate.