Study on extraction of soluble calcium from pig bone residue and preparation of chelated peptide calcium compound

[Objective] The process of extracting soluble calcium from pig bone residue was optimized. And then chelated peptide calcium compound was prepared using extracting soluble calcium. [Method] Factors affected the extraction process including acid type,amount of hydrochloric acid,solvent to material ratio,extraction temperature and extraction time,was selected and processing parameters of were optimized by the orthogonal experimental design. Finally,the affected factors in the preparation of peptide chelated soluble calcium compound including ratio,p H value,reaction temperature and reaction time,were studied. [Results] The best extraction process parameters of soluble calcium from pig bone residue were hydrochloric acid 2m L / g,solvent to material ratio 10 m L / g and extraction time 40 min.Under the optimized extraction conditions,the extraction yield was 15. 01%. The best process parameters were: ratio of soluble calcium and peptide enzyme solution were 1 ∶ 4,p H value 8,reaction temperature 50℃ and reaction time10 min. Under the optimized chelation conditions,the actual chelating rate was 95%. [Conclusion] The extraction of soluble calcium from pig bone residue with hydrochloric acid and the preparation of peptide chelated soluble calcium compound using soluble calcium from pig bone residue were feasible.