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A Study on Optimizing Extraction Process for the Ginsenoside by Orthogonal Design

Shi Li-xia

Abstract

ObjectiveTo study the best extraction process of ginsenoside.MethodsThe optimal extraction process was selected by the orthogonal design.The cotnents of ginsenoside Rg_1 and ginsenoside Re in ginseng were determined by HPLC.ResultsThe concentration of alcohol and extraction times showed significant influence on extract obtained of ginsenoside.ConclusionThe best extraction process of ginsenoside is A_2B_2C_2D_3 respectively adding 75% alcohol into crude ginseng(8∶1 in weight) and extracting 3 times for 1.5 hours each extraction.

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Key concepts: Ginsenoside, Extraction (chemistry), Ginseng, Chromatography, Chemistry, Process (computing), Alcohol, High-performance liquid chromatography