Detection and analysis of beta-lactamases on multi-resistant Pseudomonas aeruginosa

Objective To study the production of beta-lactamases on multi-resistant Pseudomonas aeruginosa, and provide the guideline for treatment and control of Pseudomonas aeruginosa infection in hospital. Methods 50 strains of multi-resistant Pseudomonas aeruginosa were selected with K-B susceptibility method, The three-dimensional method was taken to differentiate the various beta-lactamases, 2-mercaptopropanoic acid inhibited detection was taken to metallo-beta-lactamases at the same time. Results Among 50 strains of multi-resistant Pseudomonas aeruginosa, there were 2 strains (4%) producing ESBLs, 20 strains(40%)producing AmpC beta-lactamases, and 11 strains(22%) producing ESBLs and AmpC beta-lactamases at the same time. 8 strains of them was producing metallo-beta-lactamases. Conclusions The main beta-lactamases was AmpC beta-lactamases in the multi-resistant Pseudomonas aeruginosa cultured in our area. In order to reduce the drug-resistance strains and control the infection of Pseudomonas aeruginosa, antibiotics should be used reasonably according to drug susceptibility test.