Optimization of Cellulase Assisted Extraction Total Flavonoids fromBroccoli Using Response Surface Method

【Objective】The aim of the study was to extract total flavonoids fromBroccoli through optimizing cellulose process.【Method】Based on single-factor experiments,enzyme amount,pH,enzymolysis temperature and enzymolysis time were set as independent variables and the yield of total flavonoids was response value.Effects of each variable and their interactions on total flavonoids were determined using Box-Behnken method.The quadratic polynomial regression equations between total flavonoids and each variable were built using Design-Expert software.【Results】The results showed that the optimal extraction conditions of total flavonoids fromBroccoli were:enzyme amount 0.84%,pH 5.0,enzymolysis temperature 50℃,and enzymolysis time 57 min.The yield of total flavonoids was 1.251% under the conditions.The relative error was 0.556%compared to predictive value,indicating the model fitted well with experimental data.In addition,the extraction yield was increased by 41% as compared to the traditional extraction method.【Conclusion】The results could provide a theoretical foundation and scientific basis for the development of natural antioxidant products of Broccoli.