Optimization of extracting condition of total flavonoids from citrus peel by cellulose and methanol solution

In order to increase the extraction efficiency of total flavonoids from citrus peel,the extracting condition of cellulase cooperated with methanol technique was optimized by response surface methodology based on single factor experiments.The effects of the ratio of cellulase to citrus peel,enzymolysis time,enzymolysis temperature and pH value of the enzymolysis buffer on the yield of total flavonoids were studied,and a mathematical model was established and analyzed to describe the relationships between the studied factors and the extraction yield of total flavonoids.The result showed that the optimal extraction conditions was as follows: the ratio of cellulase to citrus peel 0.6%,enzymolysis time 3 h,enzymolysis temperature 63℃,pH value of enzymolysis buffer 3.7.Under the optimized extraction conditions,the total flavonoids yield was 9.87 mg/g,which was close to the estimated value(9.92 mg/g) attained from regression model.