The clinical significance of T lymphocytic subgroups and interleukin-6,interleukin-8 levels in peripheral blood of neonatal hypoxic ischemic encephalopathy

Objective To study the changes of T cell subgroups and interleukin (IL) 6,IL 8 levels in peripheral blood of neonates with hypoxic ischemic encephalopathy (HIE),the relationship between brain injuries of HIE and the parameters,and their clinical significance Methods Peripheral blood samples of 50 HIE cases and 30 normal neonates were collected.Immunophenotypes of T cell were determined by double immunofluorescence labeled assay with flow cytometry The levels of IL 6 and IL 8 were determined by enzyme linked immunosorbent assay (ELISA) Clinical data of HIE neonates were collected and analysed Results Peripheral blood CD3 + ,CD4 + ,CD8 + T cell levels in HIE group were significantly lower than those in normal control,while IL 6,IL 8 levels in HIE group were significantly higher than those in normal control The levels of CD3 + ,CD4 + ,CD8 + T cell and IL 6 were significantly related to disease severity and different prognostic cases,i e ,patient who had lower CD3 + ,CD4 + ,CD8 + levels but a higher IL 6 level might be more serious and of worse prognosis Conclusion Immune function in neonatal HIE was impaired The levels of CD3 + ,CD4 + ,CD8 + T cell and IL 6,IL 8 could be markers for assessing the disease severity and predicting outcome