Study on the relationship between the neonates with hypoxic ischemic encephalopathy and cellular immune function

Objective To study the changes of interlukinβ(IL-1β),interlukin-10 (IL-10) levels and T cell subsets in neonates with hypoxic ischemic encephalopathy (HIE) and its role in pathogenesis of HIE.Methods The levels of IL-1β,IL-10 in serum of the newborns with HIE and normal controls were dynamically detected by ELISA in the first,third,seventh day after birth.The peripheral blood mononuclear cells of newborns with HIE and normal controls were isolated by routine Ficoll-Hypaque,and the levels of CD+_3,CD+_4,CD+_8 were detected by biotin-streptavidin on spot of the first,thind and seventh day.Results The levels of IL-1β,IL-10 were higher in serum of newborns with HIE than those of normal controls (P0.05,P0.01) and were associated with severity of HIE.The levels of CD+_3,CD+_4,CD+_8 in the peripheral blood were higher in serum of newborns with HIE than those of normal controls (P0.01).There was the most abnormal cellular immune function in newborns with severe HIE.Conclusion The certain disorder of cellular immune function can be existent in newborns with HIE.IL-1β,IL-10 and T cell subsets can play a role in pathogenic course of HIE.