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Immobilization of β-glucosidase on Chitosan Beads

Shiyuan Yu

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Abstract

β-Glucosidase was immobilized on chitosan beads by adsorption and crosslinking.The method of orthogonal test was used to investigate the optimal condition of immobilization.The optimal condition of immobilization was as follows:β-glucosidase dosage 48.6 IU/g dry chitosan was absorbed with chitosan beads at pH value 5.0 for 12 h,followed by crosslinking at 25 ℃ for 2 h in 1.0 % glutaraldehyde(GA)solution.The activity of immobilized β-glucosidase and recovery rate of β-glucosidase activity were 41.76 IU/g(based on dry chitosan)and 85.93 %,respectively.

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What this paper is about

β-Glucosidase was immobilized on chitosan beads by adsorption and crosslinking.The method of orthogonal test was used to investigate the optimal condition of immobilization.The optimal condition of immobilization was as follows:β-glucosidase dosage 48.6 IU/g dry chitosan was absorbed with chitosan beads at pH value 5.0 for 12 h,followed by crosslinking at 25 ℃ for 2 h in 1.0 % glutaraldehyde(GA)solution.The activity of immobilized β-glucosidase and recovery rate of β-glucosidase activity were 41.76 IU/g(based on dry chitosan)and 85.93 %,respectively.

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Available abstract

β-Glucosidase was immobilized on chitosan beads by adsorption and crosslinking.The method of orthogonal test was used to investigate the optimal condition of immobilization.The optimal condition of immobilization was as follows:β-glucosidase dosage 48.6 IU/g dry chitosan was absorbed with chitosan beads at pH value 5.0 for 12 h,followed by crosslinking at 25 ℃ for 2 h in 1.0 % glutaraldehyde(GA)solution.The activity of immobilized β-glucosidase and recovery rate of β-glucosidase activity were 41.76 IU/g(based on dry chitosan)and 85.93 %,respectively.

Key concepts: Chitosan, Chemistry, Glutaraldehyde, Adsorption, Chromatography, Nuclear chemistry, Immobilized enzyme, Enzyme

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