Screening of fungi producing heat-resistant lassase

About 40 strains of fungi collected from Mountain Xu were screened for fungi producing high activity and heat-resistant lassase.The collected fungi were purified in bran-extract plate.The producing ability of laccase was determined by α-naphthol in the bran-extract plate.Four strains of fungi were selected for farther investigation and comparing by shaken flask cultivation.At last,the strain SY04 we named was found producing laccase most efficiently.The laccase activity of SY04 reached 330U/mL at the 3rd day of flask cultivation.The property of crude laccase produced by SY04 was investigated subsequently.As a result,the laccase produced by SY04 was heat-resistant.It could be incubated in 60℃ water about 30 minutes almost without any inactivation.The optimal temperature of catalyzing was 60℃.