Determination of Vitamin E in Vegetables Oils by Solid Phase Extraction-high Performance Liquid Chromatography

A solid phase extraction-high performance liquid chromatographic(SPE-HPLC) method was developed for the simultaneous analysis of four kinds of vitamin E in the vegetables oils.The vitamin E in the vegetables oils was extracted and purified by Alumina B SPE column.After the solid phase extraction,the purified extract was analyzed directly by high performance liquid chromatography with fluorescence detector.An excellent linearity was obtained in the range of 0.1~25 mg/L with the correlation coefficient(r) from 0.9991 to 0.9996;the limit of detection was in the range of 0.019~0.033 mg/kg;the mean extraction recoveries were in the range of 88.1-99.2% with the relative standard deviations(RSD) from 1.39 % to 3.95 % in three different vegetables oils matrixes.It was a simple and rapid method with satisfactory repeatability and recovery to determinate Vitamin E in vegetable Oils.