Preparation and Immunity Effect Assessment of an Inactivated Vaccine against Canine Parvovirus

The canine parvovirus strain JL0911 was isolated by our lab with some mutations in the main epitopes compared to the classical CPV strains isolated in China.This canine parvovirus strain could not be classified into CPV-2a,CPV-2b or CPV-2c.The aim of this study is to develop an inactivated vaccine with the JL0911 canine parvovirus strain.The canine parvovirus JL0911 was prepared in F81 cells and inactivated by formalin.A kind of nanometer adjuvant prepared by our lab was mixed with the inactivated canine parvovirus with the proportion of 1∶4.Dogs were vaccinated with 1.5 mL of this vaccine and the neutralizing antibody was detected by virus neutralization test in the F81 cell line.The results showed that the neutralizing antibody titers of the experimental dogs were elevated significantly after vaccinated 14 days by the inactivated vaccination.The most significant elevation was 29.This indicated that the canine parvovirus strain JL0911 was potential for developing inactivated vaccine with high performance.