2010•Acta Pratacultural ScienceRequires access
Cloning and sequence analysis of the chalcone synthase gene (CHS) from Fagopyrum dibotrys.
Hua Meng, Chenglei Li, Qi Wu, Ji‐Rong Shao, Hui Chen
Open publisher page 3 citations
Abstract
The conservative sequence of the chalcone synthase gene from Fagopyrum dibotrys 544 bp was isolated by homology cloning,and then the full-length DNA and cDNA ORF (open reading frame) sequences were cloned by genome-walking and RT-PCR,respectively.Sequence analysis showed that the CHS DNA of F.dibotrys was 1 650 bp,including one 462 bp intron.The coding region of cDNA was 1 188 bp,encoding 395 amino acids,designated as FdCHS and included the conserved sites of CHS such as active sites and substrate-binding pocket sites.The homology analysis of FdCHS with that of other plants showed that it was closely related to Rheum palmatum and Polygonum cuspidatum with 94% and 93% homology,respectively.The sequence accession number is GU169470 in GenBank.