Inhibition effect of siRNA on survivin expression in HepG2 cells

Objective To study the inhibition effect of siRNA on the survivin expression in HepG2 cells. Methods One pair of 21bp reverse repeated motifs of survivin target sequence with 9 spacer were synthesized and inserted into plasmid Psilencer2.1 to generate siRNA eukaryotic expression vector. After stable transfection into HepG2 cells , the survivin mRNA and protein expression inhibition was detected by use of RT-PCR and flow cytometer. Results The recombinant plasmid P Silencer(+)-survivin was successfully constructed. By use of RT-PCR and flow cytometer detection , survivin mRNA and protein expression inhibition ratio reached 73% and 75% respectively. Conclusion siRNA targeting survivin gene can specially suppress its expression in HepG2 cells. This provides a new method and material to the biological therapy of cancer.