EFFECT OF RNA INTERFERENCE TARGETING SURVIVIN GENE ON THE PROLIFERATION OF HEPATOCELLULAR CARCINOMA CELL LINE HepG2

Objective To study the inhibition of RNA interference targeting the expression of survivin gene, and assess its effects on proliferation of hepatocellular carcinoma cell line HepG2.MethodsThree different-surviving-gene sequence specific shRNA vectors pshRNA-survivin-323/387/52 were constructed. Transfection of shRNA vectors was performed using Lipofectmine 2000 liposome. The expression of survivin mRNA was detected by RT-PCR. The vector which could significantly down-regulate the expression of survivin was transfected into HepG2 cells by Lipofectmine 2000 liposome. Immunocytochemistry was employed to detect the expression of survivin protein after transfection. Proliferation of HepG2 cells was assessed by MTT.ResultsThe pshRNA-survivin-387 vector targeting survivin gene significantly down-regulated the expression of its mRNA in HepG2 cells as compared with that of the controls (F=18.64,q=4.293-4.925,P0.05). The other vectors targeting survivin gene had no influence on survivin gene expression (P0.05). After transfection with pshRNA-survivin-387 vector, the expression of survivin was obviously down-regulated (F=68.39;q=13.71, 14.37;P0.01) and the proliferation of HepG2 cells inhibited (F=20.06-47.65;q=11.186,12.601;P0.01). ConclusionThe pshRNA-survivin-387 vector targeting survivin gene could specifically suppress its expression and inhibit the proliferation of HepG2 cells.