Influence of Effective Part GBEE-2 of Exocarpium Ginkgo Extracts on Apoptosis and Apoptotic Pathway of Human Gastric Adenocarcinoma Cell Line SGC-7901

Objective To study the influence of effective part GBEE-2 of Exocarpium Ginkgo extracts on apoptosis and apoptotic pathway of human gastric adenocarcinoma cell line SGC-7901.Methods SGC-7901 cells were cultured in vitro,and methyl thiazolyl tetrazolium(MTT) method was performed to observe the proliferation of SGC-7901.Flow cytometry was utilized for measuring the apoptotic rate and cell cycle.The expression of Caspase-9,Fas and Survivin in the cultured supernatant was detected by enzyme-labeled immunosorbent assay(ELISA).Results GBEE-2 could inhibit the proliferation of cell line SGC-7901 cells in vitro at the concentrations of 5～160 μg·mL-1,which was dose-dependent.IC50 of GBEE-2 was 83 μg·mL-1.When cultured in vitro with GBEE-2 at the concentrations of 10～160 μg·mL-1,the apoptotic rate of SGC-7901 was elevated,the expressions of Caspase-9 and Fas protein increased,but the expression of survivin protein decreased(P 0.05 or P 0.01).Conclusion GBEE-2 can inhibit the proliferation of SGC-7901 cells via the apoptosis induction,and caspase pathway is involved in the apoptosis pathway.