To analyze the correlation between CD_3+CD_4+T lymphocyte count of 23 healthy persons and the relative time

Objective:To determine the relationship between CD3 + CD4 + T lymphocyte count of 23 healthy persons and the relative time.Methods: Mark the collected whole blood specimens of 23 healthy persons by FITC-CD4/CD8/CD3 antibody.Then take count of CD3 + CD4 + T lymphocyte by flow cytometry,respectively after blood sampled 0 hours,24 hours,48 hours,72 hours,96 hours.Analysis the test datum by the repeated measurement data analysis of variance through statistical software SPSS 13.0.Then test the variance between the 1st and other four times by the GLM procedure to assess the best time for the detection of CD3 + CD4 + T lymphocyte count.Results: It results from Mauchly′s Sphericity Tests that correlation exists in repeated measurement datum(P0.05).There is statistically significant between the1st and all the other four times,which depends on the results of the GLM procedure adjusted by Greenhouse-Geisser(P0.05).The droop rate between the posterior four times and the 1st are respectively 1.0%、1.8%、21.5%、31.9%.Conclusion: It is more reliable that the result comes from the flow cytometry which completed in 48 hours for CD3+CD4+T lymphocyte counts.