Optimal Extraction Conditions of Collagen from Jellyfish
Jin Gui-fen
Abstract
Jin Gui-fen
Abstract
Jellyfish is a potential resource for collagen extraction. In this paper, two methods named as acid extraction and pepsin extraction were used to extract collagen from Jellyfish, the effects of different sorts of acid solution, pepsin concentration, ratio of material to liquid and extraction time on extraction yield were researched, and the optimization of extraction conditions were examined. The results showed the optimal condition of acid extraction was using hydrochloric acid, ratio of material to liquid 1:2.0, extraction time 84h, and that of pepsin extraction was using 3.0 % of pepsin, ratio of material to liquid 1:2.0, extraction time 48h. Meanwhile, the extraction yield of pepsin extraction was higher than acid extraction.
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Jellyfish is a potential resource for collagen extraction. In this paper, two methods named as acid extraction and pepsin extraction were used to extract collagen from Jellyfish, the effects of different sorts of acid solution, pepsin concentration, ratio of material to liquid and extraction time on extraction yield were researched, and the optimization of extraction conditions were examined. The results showed the optimal condition of acid extraction was using hydrochloric acid, ratio of material to liquid 1:2.0, extraction time 84h, and that of pepsin extraction was using 3.0 % of pepsin, ratio of material to liquid 1:2.0, extraction time 48h. Meanwhile, the extraction yield of pepsin extraction was higher than acid extraction.
Key concepts: Extraction (chemistry), Pepsin, Jellyfish, Hydrochloric acid, Yield (engineering), Chromatography, Chemistry, Materials science