ScholarLens Back to search
2008•Strait Pharmaceutical JournalRequires access

Determination of chlorzoxazone in serum by RP-HPLC

Xie Tao-yin

Open publisher page 0 citations

Abstract

OBJECTIVE To establish a RP-HPLC method for the determination of chlorzoxazone in serum.METHODS Diamonsil C18(250×4.6mm,5μm) column was used.The mobile phase consisted of acetonitrile-0.02M buffer phosphate(9∶11,pH6.3) and 0.16% triethylamine,the flow rate was 1.0mL·min-1.The detection wavelength was 280nm.The column temperature was kept at 30℃.RESULTS The linear range of chlorzoxazone in serum was 2.5～12.5μg·mL-1.The limit detection was 0.4μg·mL-1.And the average recovery of the method was 102.82±4.39%.CONCLUSION The method is simple,rapid,sensitive,and reliable,and it is suitable for the determination of chlorzoxazone in serum.

About this research paper

What this paper is about

OBJECTIVE To establish a RP-HPLC method for the determination of chlorzoxazone in serum.METHODS Diamonsil C18(250×4.6mm,5μm) column was used.The mobile phase consisted of acetonitrile-0.02M buffer phosphate(9∶11,pH6.3) and 0.16% triethylamine,the flow rate was 1.0mL·min-1.The detection wavelength was 280nm.The column temperature was kept at 30℃.RESULTS The linear range of chlorzoxazone in serum was 2.5～12.5μg·mL-1.The limit detection was 0.4μg·mL-1.And the average recovery of the method was 102.82±4.39%.CONCLUSION The method is simple,rapid,sensitive,and reliable,and it is suitable for the determination of chlorzoxazone in serum.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To establish a RP-HPLC method for the determination of chlorzoxazone in serum.METHODS Diamonsil C18(250×4.6mm,5μm) column was used.The mobile phase consisted of acetonitrile-0.02M buffer phosphate(9∶11,pH6.3) and 0.16% triethylamine,the flow rate was 1.0mL·min-1.The detection wavelength was 280nm.The column temperature was kept at 30℃.RESULTS The linear range of chlorzoxazone in serum was 2.5～12.5μg·mL-1.The limit detection was 0.4μg·mL-1.And the average recovery of the method was 102.82±4.39%.CONCLUSION The method is simple,rapid,sensitive,and reliable,and it is suitable for the determination of chlorzoxazone in serum.

Key concepts: Chlorzoxazone, Chromatography, Chemistry, Triethylamine, High-performance liquid chromatography, Detection limit, CYP2E1, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of chlorzoxazone in serum by RP-HPLC — Research Paper | ScholarLens