2002•Journa of Henan Medical UniversityRequires access
Determination of chlorzoxazone in human plasma by RP-HPLC
Weifang Zhang
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Abstract
Aim: To explore an efficient method for determining the plasma concentration of chlorzoxazone. Methods: RP HPLC method was used for determining the concentration of chlorzoxazone in plasma with RP C 18 used as the stationary phase and a solution composed of methanol and water (2∶3, V ∶ V ),as mobile phase with a flow rate of 1.0 ml/min and a wavelength of 282 nm. The retention time of chlorzoxazone and phenacetin which was used as an internal standard were 5.42 and 3.94 min respectively. Results: The limit of detection was about 0.015 μg. The linear was 1.0～75 mg/L. The average recovery of the method was 98.36%. The RSD was lower than 3.5 %. Conclusion: This method is simple and precise for determining the plasma concentration of chlorzoxazone.