Reverse of Bleomycin-induced Pulmonary Fibrosis of Rats by the Effects of Resveratrol

Objective To observe the reverse of bleomycin-induced pulmonary fibrosis of the rat by the effects of resveratrol.Methods Sixty male SD rats were randomly divided into control group,model group,and middle(50 mg/kg) ,high(100 mg/kg) resveratrol-treated groups.Rat pulmonary fibrosis was reproduced by an intratracheal injection of bleomycin to observe the inflammation and fibrosis in lung tissue changing,detect the hydroxyproline content in the lung tissues,and RT-PCR was used for determination of TGF-β1 mRNA in rats.Results The model group on the 17th day,in rat lung tissue,showed typical pulmonary fibrosis in the use of RES intervention group than in model group,the degree of fibrosis change reduced with time;In the model group(HYP content of 751.80 ± 22.5,996.00 ± 39.48,1 197.60 ± 105.98,P 0.05) was significantly higher than that of the control group(266.00 ± 24.08,307.00 ± 16.40,318.00 ± 18.7,P 0.05) ;High dose of RES in the intervention group at each time point HYP content(657.45 ± 18.31,514.34 ± 9.78,504.20 ± 13.16) were compared with the model group(751.80 ± 22.52,996.00 ± 39.48,1197.60 ± 105.98) decreased(P 0.05) ;The model group TGF-β1 mRNA content on the 17th day was highly expressed than the control group,the use of high dose of RES after the intervention of TGF beta1 mRNA all time points(0.788 ± 0.205,0.472 ± 0.199,0.474 ± 0.310) than those in the model group(1.000 ± 0.782,0.759 ± 0.226,0.603 ± 0.147) .Conclusion RES may reverse the pulmonary fibrosis of rats through the regulation of TGF-β1.