Inhibition of Vitamin A on hela cells and its molecular mechanism

To investigate the effects of Vitamin A on proliferation of Hela cells and its related molecular mechanism,cell growth is observed by clony-forming test and nude mice carcinogenic test.The morphologic and hiochemical features and the percentage of apoptosis in Hela cells are performed by electron microscope,flow cytometry(FCM) and DNA agarose gel electrophoresis.Clony-forming test shows that Vitamin A can inhibit the proliferation of Hela cells(P0.01).Nude mice carcinogenic test shows Vitamin A can inhibit the growth of Hela cells in the mice(P0.01).It is found that Hela cells have obvious morphological changes including cell pykermis,nuclear membrance twist,nuclear chromosome assembling to lump and closing to membrance under electron microscope.Typical DNA ladder appears in Vitamin A-treated cells in DNA agarose gel electrophoresis.Detected by FCM,subdiploid nuclear peak appears on the left of G1 peak,diploid cells peak is reduced.The apoptotic percentage of Hela cells is positively correlated to the concentration and the time of the action of Vitamin A when the concentration of Vitamin A is between 4～8 μg/ml.Vitamin A can inhibit the proliferation of Hela cells by induction of cells apoptosis in concentration-and time-dependent manners.