2009•Journal of Zhejiang Ocean UniversityRequires access

Isolation and Identification of Causative Pathogen for Visceral White Spot in Lateolabrax japonicus Stocked in Sea-cage

Xie Jian-jun

Abstract

A serious and infectious epidemic of Lateolabrax japonicus cultured in net-cage in Xiangshan,Ningbo,Zhejiang Province,broke out from May to June 2007. From the diseased fish of natural and artificial-infections,we isolated strains LY-1 and LY-2 respectively. By the means of morphological,physiological and biochemical tests and 16S rRNA sequence analysis,we initially identified both LY-1 and LY-2 as Vibrio harveyi. HSP60 gene sequences showed that strains LY-1 and LY-2 had sequence identity of 99% to harveyi,whereas had less than 91% identity to the other vibrios. Based on the results obtained,the strains LY-1 and LY-2 were identified as Vibrio harveyi.

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Key concepts: Lateolabrax, Vibrio harveyi, Biology, Microbiology, Pathogen, 16S ribosomal RNA, Cage, Vibrio