The protective effect of Edaravone on apoptosis of cerebral neuron after cardiopulmonary resuscitation in rats

Objective:To observe the protective effects of Edaravone on neuron of brain after cardiopulmonary resuscitation(CPR) in rats.Method:Cardiac arrest was induced by asphyxiation and ice-cold KCl(0.5 mol/L).Resuscitation started 5min after cardiac arrest.Thirty six Sprague-Dawley rats were randomly divided into 3 groups:sham operation group,conventional treatment group and Edaravone intervention group(n=12).Using the colorimetry,we analyzed the quantity of maleic dialdehyde(MDA) and enzyme activity of super oxide dismutase(SOD) of the brain tissues in all groups.The apoptosis of cerebral neuron were confirmed by TUNEL.Result:Compared with the conventional treatment group,quantity of MDA declined greatly(P0.05),while the activity of SOD in cerebral neuron increased markedly in the Edaravone intervention group(P0.05).The number of apoptotic neuron in the Edaravone intervention group was fewer than the conventional treatment group.Conclusion:Edaravone will play very positive role in the protection of cerebral neuron in rats after CPR.