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STUDY ON ISOLATION, SELECTION OF CELLULOSE-DECOMPOSING MIXED STRAINS AND THE CONDITIONS FOR CELLUASE PRODUCTION

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Abstract

Two strains with considerable capability of cellulosed gradation were selected from compost. One strain is Thermomonospora sp. Q-0 and the other is Bacillus sp. Q-3. We investigated the conditions for the formation of cellulase of the Q-0?Q-3 and the mixed strains, which contains both of the strains. The result show mixed strains can degrade cotton and filter paper more effectively than Q-0 and Q-3, the degradation rates were 69% and 62% respectively. The suitable temperature for cellulase is 50 ℃ and pH is 7.5. Cotton can induce the cellulase formation, the cellulase activity of mixed strains was 100 IU/ml, almost 40 IU/ ml higher than Q-0. Inaddition,Q-0?Q-3 and mixed culture take the priority on organic nitrogen sources to inorganic nitrogen sources.

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Two strains with considerable capability of cellulosed gradation were selected from compost. One strain is Thermomonospora sp. Q-0 and the other is Bacillus sp. Q-3. We investigated the conditions for the formation of cellulase of the Q-0?Q-3 and the mixed strains, which contains both of the strains. The result show mixed strains can degrade cotton and filter paper more effectively than Q-0 and Q-3, the degradation rates were 69% and 62% respectively. The suitable temperature for cellulase is 50 ℃ and pH is 7.5. Cotton can induce the cellulase formation, the cellulase activity of mixed strains was 100 IU/ml, almost 40 IU/ ml higher than Q-0. Inaddition,Q-0?Q-3 and mixed culture take the priority on organic nitrogen sources to inorganic nitrogen sources.

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Available abstract

Two strains with considerable capability of cellulosed gradation were selected from compost. One strain is Thermomonospora sp. Q-0 and the other is Bacillus sp. Q-3. We investigated the conditions for the formation of cellulase of the Q-0?Q-3 and the mixed strains, which contains both of the strains. The result show mixed strains can degrade cotton and filter paper more effectively than Q-0 and Q-3, the degradation rates were 69% and 62% respectively. The suitable temperature for cellulase is 50 ℃ and pH is 7.5. Cotton can induce the cellulase formation, the cellulase activity of mixed strains was 100 IU/ml, almost 40 IU/ ml higher than Q-0. Inaddition,Q-0?Q-3 and mixed culture take the priority on organic nitrogen sources to inorganic nitrogen sources.

Key concepts: Cellulase, Cellulose, Compost, Chemistry, Filter paper, Strain (injury), Food science, Isolation (microbiology)

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STUDY ON ISOLATION, SELECTION OF CELLULOSE-DECOMPOSING MIXED STRAINS AND THE CONDITIONS FOR CELLUASE PRODUCTION — Research Paper | ScholarLens