Genetic polymorphism of KAP13-1 gene of four cashmere goat populations in China

【Objective】 The objectives of this study were to analyze the genetic variations of KAP13-1 gene of Hexi cashmere goat,Inner Mongolia cashmere goat,Liaoning cashmere goat and Longdong cashmere goat,and provide data for future studies on cashmere traits.【Method】 KAP13-1 genes were amplified using PCR-single stranded conformational polymorphism(PCR-SSCP) analysis to detect genetic variations in 721 cashmere goats(Hexi cashmere goat,Inner Mongolia cashmere goat,Liaoning cashmere goat,Longdong cashmere goat) in China.Then we constructed UPGMA tree by genetic distances(DA).【Result】 The PCR amplification obtained KAP13-1 gene with 491 bp,and six genotypes were revealed from the four cashmere goat populations.Five nucleotide polymorphism sites,which mainly came from point mutation,were identified in four alleles.There were three transition sites(60%) and two transversion sites(40%).The mutation of 170 and 197 T→G,C→G resulted in Leu→Arg and Thr→Ser missense mutation.Four alleles were found in Hexi and Longdong cashmere goat populations,while there was no alleles C and D in Inner Mongolia cashmere goats,and allele D had not been detected in Liaoning cashmere goat populations.Clustering results showed that Hexi and Longdong cashmere goat populations clustered together firstly,and then Liaoning and Inner Mongolia cashmere goat populations clustered together.【Conclusion】 All of the four cashmere goat populations are moderately polymorphic in KAP13-1 gene,and there are rich genetic diversities among the four cashmere goat populations.Ecological factors have certain effects on the evolution of these four cashmere goat breeds.