Chemotactic effects of serum from rats with fracture on mesenchymal stem cells derived from different sources

Objective To compare the morphology of mesenchymal stem cells(MSCs)from bone marrow with from peripheral blood and investigate the chemotactic effects of serum from rats with fracture on MSCs derived from different sources. Methods Thirty-six Sprague-Dawley rats were randomly divided into 2 groups:fracture group(n=24,of with MSCs from peripheral blood were cultured from 12 rats,serum was separated from others),control group(n=12).Bone marrow and peripheral blood of rats in both groups were collected to isolate and culture MSCs.Growth speed and cell morphology of MSCs were observed.Effects of both serums on chemotaxis of MSCs derived from different sources and their migration ability were tested with a Transwell system.Results MSCs were successfully obtained from bone marrow of all rats in both groups,but only from peripheral blood of 9 rats with fracture(100%vs 75%).There was no obvious difference in the cell morphology between the both groups.The number of MSCs migrating to the lower surface of Transwell plate after treatment with serum from rats with fracture was significantly higher than that after the treatment with control rat serum(P0.01).The migration rate of MSCs which were derived from bone marrow in control group was obviously lower than those derived from bone marrow and peripheral blood from rats with fracture(P0.01).Difference of the migration ability existed between MSCs derived from bone marrow and peripheral blood in fracture group(P0.01).Conclusions Serum from rats with fracture has a stronger chemotactic effect on MSCs. Moreover,the migration ability of MSCs derived from rats with fracture is stronger than that of MSCs derived from control rats, while the migration ability of MSCs from peripheral blood of rats with fracture is the strongest.