Effect of Bone Marrow Mesenchymal Stem Cells on Serum Biochemical Indexes of Rats with Pancreas Tissue Injury

Objective To observe the effect of bone marrow mesenchymal stem cells(MSCs)on the serum biochemical index-es of rats with pancreas tissue injury. Methods The rat model of pancreas tissue injury was established by ligating pancreas. The MSCs of healthy rats were isolated, purified and cultured in vitro, then determined for cell cycle and surface markers by flow cytome-try. The model rats were injected i.v. with MSCs and observed daily, and their serum samples were collected 24 h, 48 h, 7 d and 15 d after treatment for determination of amylase and lipase. Results Of the isolated and purified MSCs, 86.67% were at G0 / G1 phase. The expression of surface marker CD44 was observed on the surface of MSCs, but no expression of hemopoietic stem cell surface marker CD34. Visible observation showed that the appearance of necrotic pancreas tissue of rats recovered to normal 15 d after treat-ment with MSCs. The serum amylase contents of rats 24 h, 48 h and 7 d after treatment with MSCs were significantly lower than those of model rats untreated, while showed no significant difference 15 d after treatment. The serum lipase content of rats 24 h, 48 h, 7 d and 15 d after treatment with MSCs were significantly lower than those of model rats untreated. However, both the serum amylase and lipase contents of rats at any time points after treatment with MSCs were significantly higher than those of healthy rats. Conclusion MSCs showed curative effect on pancreas tissue injury in rats.