Research of Regeneration System on Cherry Rootstock of Gisela

Through the studies of the induction and differentiation of leaves and petioles of two varieties of cherry rootstock,we got the conclusion that the differentiating ability of petioles is more efficient than the leaf-discs,BA does better than KT,NAA is more effective than IBA.From all these factors considered,we get the a formula used both for shoot induction and subculture:MS+BA 1～2 mg/L+KT 1～2 mg/L+NAA 0.5 mg/L+IBA 0.5 mg/L.The highest frequency of shoot induction reaches 42%～67%.In the medium of 1/2MS with ABT 2 mg/L,the rooting rate can reach 91.8% and the survive rate can reach 93.8%.