Study on Tissue Culture and Rapid Propagation Technology of Big Sweet Cherry Rootstock Gisela

[Objective] The research aimed to establish the tissue culture and rapid propagation technology system of big sweet cherry rootstock Gisela.[Method]With the shoot apical meristem of Gisela No.5 as material,the paper studied the tissue culture and rapid propagation technology from axillary buds induction,subculture,rooting and plantlet hardening and transplantation.[Result]The results showed that the optimum medium for axillary buds induction was MS+6-BA 1.00 mg/L +NAA 0.03 mg/L +KT 1.50 mg/L +GA3 0.20 mg/L;1/2 MS+IBA 1.50 mg/L was suitable for the test tube plantlet rooting.The young plantlet had high survival rate when took the 1/2 straw dung and 1/2 garden soil as transplantation substrate.[Conclusion]This study will provide the theoretical and practical basis for rapid production of tissue culture of Gisela No.5.