Toxic Effects of Proliferation on Skin Fibroblasts Induced by Inorganic Arsenic

Objective To study the toxic effects of inorganic arsenic on skin fibroblasts proliferation,and to explore the molecular mechanism of sodium arsenite for its role in skin lesions from arsenic-contamination.Methods Cell number counting,MTT reduction assay,transmission electron microscope were performed in skin fibroblasts,and sodium arsenite was used as tester for inorganic arsenics.Results With different concentration of sodium arsenite 0.5～5.0 μmol/L,the skin fibroblasts were markedly stimulated,and a dose effect relationship was observed.At the concentration of 10.0 μmol/L,however,sodium arsenite induced injury of skin fibroblasts.Conclusions The proliferation of the skin fibroblasts is regulated in a double-phase manner by sodium arsenite.Low concentration induces stimulation effect,but high concentration inhibits the cell proliferation.