Effects of proliferation on primary cultured human skin cells induced by sodium arsenite

Objective: To investigate the effects of injury on primary cultured skin cells induced by sodium arsenite. Methods: Count cell number, MTT reduction assay,transmission electron microscope were performed in primary cultured skin cells, sodium arsenite was used as tester. Results: With different concentrations of 0.5～8 μmol/L sodium arsenite, the skin fibroblasts were markedly stimulated and showed a dose effect relationship. Sodium arsenite at lower concentrations (0.8 μmol/L?1.6 μmol/L) induced mainly the degree of proliferation of epidermic cell, while inhibiting cell growth over 3.2 μmol/L. At concentration of 10 μmol/L, however, sodium arsenite induced injury of skin fibroblasts. Conclusion: The proliferation of skin cell may play a critical role in the development of skin injury caused by inorganic arsenic, the mechanism of DNA injury in primary cultured human skin cells needs further study.