QTL Mapping of Protein and Oil Content in Soybean

A population consisted of 96 recombinant inbred lines of soybean was developed from an intraspecies cross between Glycine max,TK780 and Glycine soja,Hidaka4.A genetic linkage map of soybean genome had been constructed.MapQTL5.0 multiple-QTL model and Excel 2007 were used to identify quantitative loci(QTLs)associated with protein and oil content in the RIL population,and analyze the relationship between seed protein and oil content.The results indicated that there were a negative phenotypic correlation between protein and oil content,and two QTLs related to seed protein content were identified on E and I linkage groups,explaining 15.6% and 21.1% phenotypic variance,respectively;three QTLs related to seed oil content were identified on E,H and I linkage groups,explaining 21.1%,17.2% and 28.0% phenotypic variance,respectively.In addition,two QTLs were identified which controlled seed protein and oil content simultaneously on E and I linkage groups.