Effects of Carbenoxolone on Upregulation of Connexin 32 in Hippocampus of Pentylenetetrazole Kindled Rats

OBJECTIVE To investigate the effects of carbenoxolone on upregulation of connexin 32(CX32) in the hippocampus of kindled rats.METHODS Before the kindled model was induced by pentylenetetrazole,some rats were administered with carbenoxolone(10 mg·kg~(-1) or 20 mg·kg~(-1)).The hippocampal tissue was used to measure the level of CX32 by western blot technique.RESULTS The expression of CX32 was elevated significantly in kindled rats(P0.05) but was not increased in 20 mg·kg~(-1) carbenoxolone treated group as compared with the controls.The seizure stages were not inhibited by 10 mg·kg~(-1) carbenoxolone but decreased significantly in 20 mg·kg~(-1) carbenoxolone treated group(P0.01).The latency in carbenoxolone treated group was prolonged in a dose dependent manner.CONCLUSION The increases of the expression of CX32 might be associated with epilepsy kindling and gap junction blocker.Carbenoxolone plays a certain protective role on seizure development.