Effects of X-linked inhibitor of apoptosis protein on cultured cardiomyocytes of neonate rat apoptosis induced by hypoxia/reoxygenation

Objective:To study whether X-linked inhibitor of apoptosis protein (XIAP) could suppress apoptosis which induce apoptosis of cardiomyocytes by transfected with pDsRed2-XIAP,inorder to search for new gene therapy for ischemic heart diseases. Method:The cardiocytes of neonate SD rat were cultured for four days. To set up fourstudy groups: ①the XIAP group:pDsRed2-XIAP plasmid was transfected into cadiocytes by liposomes,after 48 hours,then making hypoxia for 2hs and reoxygenation for 1 h;②the preconditioning group : firstly makingprecondition then carrying out 2 h of hypoxia and 1 h of reoxygenation;③the hypoxia/reoxygenation group:directly making cardiocytes hypoxia for 2h and reoxygenation for 1h;④the normoxia group: to make cardiocytes incubate for 3h in CO2. Lastly,each group were detected apoptosis rates of myocardial cell by Annexin V FITC. The difference of each group was analyced by one-way ANOVA. Result:①pDsRed2-XIAP plasmid can be transfected into cardiomyocytes by liposomes. ②Compared with the hypoxia/reoxygenation group,cardiomyocytes apoptosis rates in the XIAP group and the preconditioning group obviously decrease with statistics significance.③Cardiomyocytes apoptosis rate in the XIAP group and the pretreatment group are similar without statistics significance.Conclusion:Apoptosis of cardiomyocytes could be induced by hypoxia for 2 h and reoxygenation for 1 h XIAP. The cell apoptosis regulatory factor,can obviously reduce cardiomyocytes apoptosis induced by hypoxia/reoxygenation in newly-born rat. The effect of inhibiting apoptosis is similar between X-linked inhibitor of apoptosis protein and hypoxia-pretreatment.