2007•Zhongguo yaofangRequires access
Effects of Prostaglandin E_1 on Hypoxia/Reoxygenation Apoptosis and the Expression of bcl-2 and bax in Cardiocytes of the Neonate Rats
Xiang‐qin Ma, Shi-Ai Weng, Lijie Song
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Abstract
OBJECTIVE:To study the action mechanism of prostaglandin E1(PGE1)in the inhibition of hypoxia/reoxygenation apoptosis of cardiomyocytes in the cultured neonatal rats.METHODS:The hypoxia/reoxygenation model was made with the neonatal rat cardiomyocytes cultured by ex vivo purification,with drugs administered in PGE1(high,medium and low dosages)groups during hypoxia/reoxygenation.Hypoxia/reoxygenation apoptosis was studied by electron microscope and in situ end-labeling(ISEL).The expressions of bcl-2 and bax were detected by immunochemistry technique.RESULTS:In PGE1(high,medium and low dosages)groups compared with model group,the rate of apoptosis of cardiomyocytes decreased significantly,the expression of bac-2 increased significantly while the expression of bax decreased significantly(P0.01).CONCLUSION:Hypoxia/reoxygenation injury model established by ex vivo purification culture of cardiomyocytes of neonatal rats can induce cardiomyocyte apoptosis,while PGE1 can significantly inhibit the cardiomyocyte apoptosis,the mechanisms are possibly due to the increasing of the expression of bcl-2 and inhibition of the expression of bax.