Cloning and Bioinformatic Analysis of cDNA Encoding Sequence of Bovine ME1 Gene

ME1 gene mRNA sequence was checked from GenBank of NCBI(GenBank Accession: XM-613987),any similarity of its 1-69 bp sequence with ME1 gene mRNA sequence of human,mouse and pig in GenBank could not be found,therefore,this sequence must have something wrong.The present study is using the accessed ME1 gene mRNA sequences of human as electron probe,to find out 44 ESTs related sequences of cattle,and then designed three pairs of primers based on this ESTs contig.Extracted total RNA from liver and muscle of cattle,then through cloning and sequencing,three segments were obtained that there were M1(525 bp),M2(1039 bp) and M3(1171 bp),and splicing them as a 2015 bp sequence.This sequence is identical with the ESTs contig consensus.Additionally,the similarity of this sequence in cattle ME1 gene mRNA is 89%,85% and 84% compared with corresponding sequences in human,pig and mouse,respectively.Therefore,the correctness of this sequence was confirmed.This sequence had accessed to NCBI(GenBank Accession: FJ495084).Our present study will provide more correct sequence information of bovine ME1 gene mRNA for further study on ME1 gene structure in cattle.