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2010•Zhongguo shengwuzhipinxue zazhiRequires access

Transcription of Heat Shock Protein 47 mRNA in Rats with Acute Spinal Cord Injury

HE Yu-bao

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Abstract

Objective To investigate the transcription of heat shock protein 47(HSP47)mRNA in the rats with acute spinal cord injury(SCI).Methods Wistar rat model of SCI was established by clip-compression,setting normal rats and those receiving sham operation as controls.The Baso,Beattie,Bresnahan(BBB)Locomotor Rating Scales were assessed 3 d and every weeks,and HSP47 mRNA transcription levels in rats were determined by semi-quantitative RT-PCR 1,3,5 and 8 weeks after SCI respectively.Results The BBB Locomotor Rating Scales of rats decreased after SCI,and increased gradually as the increasing time.However,the HSP47 mRNA transcription levels increased significantly,especially at the 8th week after SCI.Conclusion The transcription level of HSP47 mRNA of Wistar rats increased after SCI,indicating that HSP47 gene might be involved in the pathogenic change of SCI.

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What this paper is about

Objective To investigate the transcription of heat shock protein 47(HSP47)mRNA in the rats with acute spinal cord injury(SCI).Methods Wistar rat model of SCI was established by clip-compression,setting normal rats and those receiving sham operation as controls.The Baso,Beattie,Bresnahan(BBB)Locomotor Rating Scales were assessed 3 d and every weeks,and HSP47 mRNA transcription levels in rats were determined by semi-quantitative RT-PCR 1,3,5 and 8 weeks after SCI respectively.Results The BBB Locomotor Rating Scales of rats decreased after SCI,and increased gradually as the increasing time.However,the HSP47 mRNA transcription levels increased significantly,especially at the 8th week after SCI.Conclusion The transcription level of HSP47 mRNA of Wistar rats increased after SCI,indicating that HSP47 gene might be involved in the pathogenic change of SCI.

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Available abstract

Objective To investigate the transcription of heat shock protein 47(HSP47)mRNA in the rats with acute spinal cord injury(SCI).Methods Wistar rat model of SCI was established by clip-compression,setting normal rats and those receiving sham operation as controls.The Baso,Beattie,Bresnahan(BBB)Locomotor Rating Scales were assessed 3 d and every weeks,and HSP47 mRNA transcription levels in rats were determined by semi-quantitative RT-PCR 1,3,5 and 8 weeks after SCI respectively.Results The BBB Locomotor Rating Scales of rats decreased after SCI,and increased gradually as the increasing time.However,the HSP47 mRNA transcription levels increased significantly,especially at the 8th week after SCI.Conclusion The transcription level of HSP47 mRNA of Wistar rats increased after SCI,indicating that HSP47 gene might be involved in the pathogenic change of SCI.

Key concepts: Messenger RNA, Spinal cord, Spinal cord injury, Heat shock protein, Transcription (linguistics), Reverse transcription polymerase chain reaction, Biology, Medicine

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