2010Chinese Journal of Trauma and Disability MedicineRequires access

Effects of IF and Ultrastructure of Rat's Smooth Muscle by Swimming Exercises

Zhengdong Chen

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Abstract

Objective:To explore the effects of IF and ultrastructure of rat's smooth muscle(SM) by swimming exercises,so as to provide some morphological basis of the changes of physiology and pathology by the exercise-induced change of smooth muscle.Methods:After exercises of EST,rat's smooth muscle were cut off.These specimens were treated respectively with regular procedures of paraffin sections and transmission electron microscope(TEM).The paraffin sections were stained by Des and Vim immunhistochemistry,their mean optics density and percentage of stained areas were observed and calculated.The ultrastructure of muscles were observed by TEM.Resuilts:In 8-week EST,Des,Vim and ultrastructure in SM of LIT had no significant changes compared with ETC.In MIT,Des and Vim of SMM increased obviously and the ultrastructure of SMM had adaptable changes.In HIT,Des and Vim decreased remarkably.At the same time,the injury changes were found in ultrastructure of SM.Conclusion:Swimming exercises with different intensity endurance would have different effects on IF and ultrastructure of SMM.

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Objective:To explore the effects of IF and ultrastructure of rat's smooth muscle(SM) by swimming exercises,so as to provide some morphological basis of the changes of physiology and pathology by the exercise-induced change of smooth muscle.Methods:After exercises of EST,rat's smooth muscle were cut off.These specimens were treated respectively with regular procedures of paraffin sections and transmission electron microscope(TEM).The paraffin sections were stained by Des and Vim immunhistochemistry,their mean optics density and percentage of stained areas were observed and calculated.The ultrastructure of muscles were observed by TEM.Resuilts:In 8-week EST,Des,Vim and ultrastructure in SM of LIT had no significant changes compared with ETC.In MIT,Des and Vim of SMM increased obviously and the ultrastructure of SMM had adaptable changes.In HIT,Des and Vim decreased remarkably.At the same time,the injury changes were found in ultrastructure of SM.Conclusion:Swimming exercises with different intensity endurance would have different effects on IF and ultrastructure of SMM.

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Available abstract

Objective:To explore the effects of IF and ultrastructure of rat's smooth muscle(SM) by swimming exercises,so as to provide some morphological basis of the changes of physiology and pathology by the exercise-induced change of smooth muscle.Methods:After exercises of EST,rat's smooth muscle were cut off.These specimens were treated respectively with regular procedures of paraffin sections and transmission electron microscope(TEM).The paraffin sections were stained by Des and Vim immunhistochemistry,their mean optics density and percentage of stained areas were observed and calculated.The ultrastructure of muscles were observed by TEM.Resuilts:In 8-week EST,Des,Vim and ultrastructure in SM of LIT had no significant changes compared with ETC.In MIT,Des and Vim of SMM increased obviously and the ultrastructure of SMM had adaptable changes.In HIT,Des and Vim decreased remarkably.At the same time,the injury changes were found in ultrastructure of SM.Conclusion:Swimming exercises with different intensity endurance would have different effects on IF and ultrastructure of SMM.

Key concepts: Ultrastructure, Anatomy, Medicine, Smooth muscle, Electron microscope, Pathology, Internal medicine, Physics

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