Study on the apoptosis of osteosarcoma cells induced by combination of TRAIL and arsenic trioxide

Objective:To study the inhibitive effect of combination of TRAIL and As2O3 on the osteosarcoma cell lines and explore the nerw therapy of osteosarcoma.Methods:TRAIL and As2O3 were used to treat the OS732 osteosarcoma cells individually or jointly and observe their effects on inducing apoptosis of osteosarcoma cells.Results:The inhibition rate was 51.32% by MTT essay which was significantly higher than the application of 50ng/ml TRAIL(9.85%) and 2μmol/L As2O3(20.36%)respectively(P0.01),after twenty-four hours of the application of 50ng/ml TRAIL and 2μmol/L As2O3 jointly on OS732 cells,we observed morphological changes with inverted phase contrast microscope,fluorescence microscope which showed tumor-killing effect of combined application was much stronger than individual application.Conclusion:Combination of TRAIL and As2O3 is obviously conducive to killing effect of osteosarcoma cells which were achieved by inducing apoptosis.