Protective Effect of Esomeprazole for Gastric Mucosa in Rats

Objective:To observe the protective effect of esomeprazole for gastric mucosa in rats.Methods:Gastric chamber perfusion with esomeprazole(20mg/kg)and intravenous injections with nitro-Larginine methyl ester(L-NAME,4 mg/kg)were administered in rats with gastric mucosal lesion induced by pure alcohol.Gastric mucosal blood flow(GMBF)was assessed with laser Doppler flowmetry(LDF),gastric mucosal NO-2/NO-3 ratio were measured by cadmium granulation reduction and colorimetric method,and the changes of ulcer index and the severity of tissues necrosis and neutrophils infiltration were observed.Results:The ulcer index of rats with esomeprazole treatnent was much lower than that of the control group(P0.01) with lesser degree of tissues necrosis and neutrophils infiltration(P0.05).The protective effect of esomeprazole was significantly inhibited by prior administration of LNAME that.Gastric esomeprazole administration obviously increased GMBF and gastric mucosal NO-2/NO3 ratio,and such effect,apart from its action against secretion,could be counteracted by pretreatment with L-NAME.Conclusion:Esomeprazole plays an important protective role in the gastric mucosa in rats,in which,nitric oxide(NO)is involved.