The Protective Effect of Omeprazole on the Gastric Mucosal Lesion in Rasts and it's Mechanisms

Gastric mucosal blood folw(GMBF)was assessed with laser Doppler flowmetry (LDF),gastric mucosal and serum NO 2/NO 3 were measured by cadmium granulation reduction and colorimetric method,and the changes of ulcer index and the severity of tissues necrosis and neutrophils infiltration were observed. The results showed that ulcer index of the omeprazole group was much lower than control group(P0 01),and the degree of tissues necrosis and meutrophils infiltration were much milder than that of the control group(P0 01).The protective effect of omeprazole was significantly decreased by prior admindistration of Nw nitro L arginine methyl ester(L NAME).The inhibitory effect of L NAME was antagonized by prior administration of L arginine. Omeprazole administered into the vein obviously increased GMBF,gastric mucosal and serum NO 2/NO 3,this effect was prevented by pretreatment with L NAME.The results suggest that omeprazole plays an important protective role on the gastric mucosal lesion in rats via nitric oxide.