Culture and osteogenic potential of bone marrow stromal cells in rats

Objective To study the rat bone marrow stromal cells (BMSCs) isolation and culture methods,and the potential of rat BMSCs differentiating into osteoblast through drug induction.Methods In this study,in 2010,using rat whole bone marrow culture method for isolation and culture of BMSCs,cell morphology was observed under light microscope,after the passage to the desired number of cells were randomly divided 2 groups,one group with drug laws (0.1μmol/L dexamethasone,10 mmol/L of sodium β-glycerophosphate,0.2 mmol/L vitamin C) for bone induction,the other group did not use other methods osteogenic induction.1,3,5 in the first day of induction,cells were two groups of samples,the alkaline phosphatase,MTT test;in the induction of the first five days scanning electron microscopy.Osteogenic cells were compared the difference in ability.Results The results of light microscopy,isolation and culture of cells were round,polygonal,spindle-shaped fibroblast-like appearance,etc.,a larger proportion of nuclear plasma,high transmittance,after continuous culture showed growth of bundle and swirling,in line with mesenchymal stem cells morphological features.Bone induction in the first day,two groups of cells,activity detection was significantly (P 0.05),alkaline phosphatase expression was no significant difference (P 0.05);in the induction of the first 3,5 days,two groups of cells,activity detection,alkaline phosphatase expression differences were statistically significant (P 0.05).Induction training in the first 5 days,making two sets of cells SEM specimens,scanning electron microscopy shows that two different cell morphology.Conclusion The whole bone marrow culture method can be effective in rat BMSCs culture and cells isolated from cell line with characteristics of mesenchymal stem;dexamethasone,β-glycerophosphate sodium,vitamin C can induce rat BMSCs differentiating into osteoblast.