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Influence of caveolin-1 on the ET-1-induced VSMC proliferation

Tan Zhi, Jianwen Chen, Jing-Yun Pan, Wang Ting-huai

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Abstract

AIM: To investigate the effect of caveolin-1 on the endothelin-1 (ET-1)-induced vascular smooth muscle cells (VSMC) proliferation. METHODS: The -thymidine (TdR) incorporation, immunofluorescence assays and western blotting were used in this study. RESULTS: The ETA receptor specific antagonist BQ123 inhibited the increase in TdR incorporation in response to ET-1 on VSMC. Immunofluorescence assays showed that caveolin-1 was mostly distributed in plasmalemma of VSMC. After 24 h treatment of VSMC with ET-1, the expression of caveolin-1 in VSMC was significantly decreased. Western blotting showed that ET-1 inhibited the expression of caveolin-1 in VSMC, BQ123 reversed the effect of ET-1. CONCLUSION: Caveolin-1 was mostly distributed in plasmalemma of VSMC. ET-1 downregulated caveolin-1 expression in VSMC via ETA receptor.

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What this paper is about

AIM: To investigate the effect of caveolin-1 on the endothelin-1 (ET-1)-induced vascular smooth muscle cells (VSMC) proliferation. METHODS: The -thymidine (TdR) incorporation, immunofluorescence assays and western blotting were used in this study. RESULTS: The ETA receptor specific antagonist BQ123 inhibited the increase in TdR incorporation in response to ET-1 on VSMC. Immunofluorescence assays showed that caveolin-1 was mostly distributed in plasmalemma of VSMC. After 24 h treatment of VSMC with ET-1, the expression of caveolin-1 in VSMC was significantly decreased. Western blotting showed that ET-1 inhibited the expression of caveolin-1 in VSMC, BQ123 reversed the effect of ET-1. CONCLUSION: Caveolin-1 was mostly distributed in plasmalemma of VSMC. ET-1 downregulated caveolin-1 expression in VSMC via ETA receptor.

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Available abstract

AIM: To investigate the effect of caveolin-1 on the endothelin-1 (ET-1)-induced vascular smooth muscle cells (VSMC) proliferation. METHODS: The -thymidine (TdR) incorporation, immunofluorescence assays and western blotting were used in this study. RESULTS: The ETA receptor specific antagonist BQ123 inhibited the increase in TdR incorporation in response to ET-1 on VSMC. Immunofluorescence assays showed that caveolin-1 was mostly distributed in plasmalemma of VSMC. After 24 h treatment of VSMC with ET-1, the expression of caveolin-1 in VSMC was significantly decreased. Western blotting showed that ET-1 inhibited the expression of caveolin-1 in VSMC, BQ123 reversed the effect of ET-1. CONCLUSION: Caveolin-1 was mostly distributed in plasmalemma of VSMC. ET-1 downregulated caveolin-1 expression in VSMC via ETA receptor.

Key concepts: Vascular smooth muscle, Caveolin 1, Blot, Endothelin 1, Immunofluorescence, Endothelin receptor, Caveolin, Receptor

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