Inhibitory Effect of K_6[P_2W_(18)O_(62)]·14H_2O on Human Breast Tumor Cell MCF-7 Growth in Vitro

OBJECTIVE: To study the in vitro anti-tumor activity of tungstophosphate compound(K6[P2W18O62]·14H2O) with Dawson structure.METHODS: MCF-7 cells were treated with different concentration of K6[P2W18O62]·14H2O,then its IC50 was measured by MTT assay;flow cytometry was applied to analyze cell cycle and apoptosis by computing the ratio of MCF-7 cells in different phase and its apoptotic ratio.Cell growth and viability were measured by trypan blue staining and cell counting by computing the ratio of living cells.The synergetic anti-tumor activity of tungstophosphate compound(K6[P2W18O62]·14H2O) and pharmorubicin was studied.RESULTS: K6[P2W18O62]·14H2O inhibited MCF-7 cell proliferation with IC50 of(33.7±3.2) μmol·L-1,it reduced the proportions of MCF-7 cells at S phase and G1 phase,with the apoptotic rate at 3.7%～29.2% within 48 h.The percentage of living cells was 95.37%～76.78% at 48 h.As compared with pharmorubicin alone,the addition of K6[P2W18O62]·14H2O to pharmorubicin showed a significantly higher inhibitory action on proliferation of MCF-7 cells(P0.05).CONCLUSION: K6[P2W18O62]·14H2O can markedly inhibit tumor cell growth,induce cell apoptosis and enhance the anti tumor effect of pharmorubicin.