Study on inhibition on human cholangiocarcinoma cell of PC in vivo and in vitro

Objective To study the inhibition effect of PC on human cholangiocarcinoma in vivo and vitro.Methods Cells were cultivated in vitro,and then were divided into positive control group,negative control group and PC group randomly after cultured for 24 h.The cytostatic effect of PC on human cholangiocarcinoma w as evaluated by MTT assay.Flow cytometry w as utilized for measuring the apoptosis and cell cycle.The nude mice w ith QBC939 cell xenografts w ere divided into 5 groups randomly: negative control group,positive control group,PC groups(three dosage groups),and the mice w ere treated w ith drugs via intraperitoneal injection daily.The tumor size w ere measured every three days.After 12 d of treatment,the tumor was weighed,and the inhibitory rate w as calculated.Results PC inhibited cell grow th in a concentration-and-time dependent manner.PC blocked cell cycle in phase S,and elevated the apoptosis rate,PC remarkably inhibited the grow th of QBC939 cell xenografts.Conclusion PC can inhibit the grow th of SHG-44 in vitro and in vivo,and induce the apoptosis of QBC939.