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Determination of Selenium in Biological Samples by Hydride Generation Atomic Fluorescence Spectrometry

Wang Yu-lin

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Abstract

A method for determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry was developed.The samples were decomposed with HNO3-HClO4 and Fe3+ was used as relieving reagent in 4 mol/L HCl medium.The detection limit of the method was 0.02 μg/L.The method has been applied to the determination of selenium in biological national standard reference materials and the results are in agreement with certified values with precision of 1.51% RSD(n=11).

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What this paper is about

A method for determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry was developed.The samples were decomposed with HNO3-HClO4 and Fe3+ was used as relieving reagent in 4 mol/L HCl medium.The detection limit of the method was 0.02 μg/L.The method has been applied to the determination of selenium in biological national standard reference materials and the results are in agreement with certified values with precision of 1.51% RSD(n=11).

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Available abstract

A method for determination of selenium in biological samples by hydride generation-atomic fluorescence spectrometry was developed.The samples were decomposed with HNO3-HClO4 and Fe3+ was used as relieving reagent in 4 mol/L HCl medium.The detection limit of the method was 0.02 μg/L.The method has been applied to the determination of selenium in biological national standard reference materials and the results are in agreement with certified values with precision of 1.51% RSD(n=11).

Key concepts: Selenium, Hydride, Certified reference materials, Chemistry, Detection limit, Reagent, Mass spectrometry, Fluorescence spectrometry

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