Comparison Analysis of Chlorogenic Acid and 3,5-O-dicaffeoylquinic Acid in Different Parts of Chrysanthemum indicum

Objective:To analyze the contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of Chrysanthemum indicum,which sources from hengyang,Hunan province.Method:Chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of C.indicum were extracted by the method of ultrasonic method with 80% ethanol and analyzed by RP-HPLC at the wavelength of 327 nm with the flow rate of 1.0 mL·min-1.Meanwhile,0.05% H3PO4 aqueous solution and acetonitrile were used as the mobile phase with gradient elution.Result: The linear range(n=5) of chlorogenic acid(r=0.996)and 3,5-O-dicaffeoylquinic acid(r=0.992) was 10-100 mg·L-1.The average recoveries(n=6) of chlorogenic acid and 3,5-O-dicaffeoylquinic acid were 98.5% and 99.7%,respectively.The contents of chlorogenic acid in the different parts of C.indicum including leaves,flowers,buds,tender stems and old stems was 1.08%,0.44%,0.39%,0.58% and 0.52%,respectively,while the contents of 3,5-O-dicaffeoylquinic acid was 1.66%,1.01%,0.82%,0.93% and 0.64%,respectively.The contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the leaves are the highest of the all tested samples.Conclusion:Different parts of C.indicum tested all have chlorogenic acid and 3,5-O-dicaffeoylquinic acid,but the contents of which was obvious difference.