Comparison of Effects of Bacille Calmette-Guerin-Polysaccharide-Nucleic Acid and Attenuated Bacille Calmette-Guerin Therapy on Children with Asthma and Changes of Cytokines from Peripheral Blood Mononuclear Cells before and after Treatment

Objective To study the functions of two different kinds of drugs on regulating TH1/TH2 of children with asthma by comparing the effects of bacille calmette-guerin-polysaccharide-nucleic acid (BCG-PSN) and attenuated BCG.Methods Study groups were selected from 60 cases of acute asthmatic children,group A(20 cases) was treated with BCG-PSN combined with GINA therapy,group B (20 cases) was treated with attenuated BCG combined with GINA therapy, and group C(20 cases) with GINA therapy alone. Levels of IL-4, IL-5, IFN-γ derived from peripheral blood mononuclear cells (PBMC) and serum IgE measured with ELISA were monitored before and 10-12 weeks after treatment.Results 1.Clinical treating effect : group A was better than group B, group B was better than group C (all P0.05). 2.Level of IFN-γ in each study groups after treatment were higher than that before treatment, le- vels of IL-4 , IL-5, IgE were lower than those before treatment. In the change of IL-5, group A was better than that of group B (P0.05) and group C(P0.01). In the changes of IL-4 , IFN-γ, IgE , there were no difference between group A and group B.Conclusions 1.The clinical effect of intramuscular injection of BCG-PSN several times is better than clinical effect with intradermal injection of attenuated BCG once.2.Vaccinating attenuated BCG once with small amount has almost the same effect as intramuscular injection of BCG-PSN several times in regulating the levels of IL-4, IFN-γ, IgE, but has not as good as the latter in regulating the level of IL-5.