Stydy of hepatic ischemia/reperfusion(I/R) injury in the rat

Objective To investigate hepatic I/R injury and the role of neutrophils in ischemia reperfused rat liver. Methods 24 healthy male SD rats were randomly divided into there groups;group A was served as sham operated control group, group B was subjected to 90min lobar, rather than totlal hepatic ischemia and group C was subjected to 120 min reperfusion after 90 min ischemia. In addition to histologic sections of liver in each group, plasma tumor necrosis factor α(TNF α),interleukin 1β(IL 1β),alanine aminotransferase(ALT), aspartate aminotransferase (AST), lactate dehydrogenase(LDH) levels and neutrophils in infiltration in liver tissue(content of myeloperoxidase) were respectively examined in A, B and C group. Results There was histologic evidence of liver injury. Plasma ALT, AST, LDH ,as evidenced by significant increase of developed after 120 min reperfusion. In group C , plasma TNF α?IL 1βlevels, as compared with A group, revealed a important elevation .The content of myeloperoxidase(MPO) in liver, a index of neutrophilic infitration in liver tissue, went up to 2 518±0 901(U/g) from 0 108±0 005(U/g) in control group, at the end of 120 min reperfusion, following 90 min of ischemia. Conclusion Proinformation cytokines such as TNF α?IL 1βmediate neutrophil induced hepatic ischemia/reperfusion injury.